Determining nail consistency by quantification of type I keratins

Nail consistency is a little studied characteristic of the nail plate. Numerous factors can influence its determination, including anatomy apparatus, structure plate, and type quantity keratins present. To study relationship between expression level I keratins, sample was chosen 32 individuals in same age group (49.94 ± 3.38 years), 18 with hard nails 14 soft nails, number for each gender. Two buffers different concentrations reducing agent (50mM 200mM) two antibodies to various were analyzed by immunoblotting. The mean extracted protein concentration at 50mM significantly higher than 200mM (p-value<0.001). obtained AE13 antibody did not vary gender or (p-values≥0.942). With cytokeratin 17 (CTK17), no differences found (p-value=0.341). However, significant established hard-consistency soft-consistency (p-values≤0.007) concentrations, (p-value=0.001). Hard-consistency had K17. Adding elemental analysis layers flexural behavior plate reported previous studies, this work demonstrates that also depends